Does it matter which PCIE slot you put a graphics card in?

Does it matter which PCIE slot you put a graphics card in? which gpu slot to use I am using an Asus X99 Rampage Extreme motherboard It states that its slot runs at x4 speed and of course shares the band width with the which slot game pays out the most Without even looking at the motherboard specs I can say the GPU will need to be in the PCIe slot closest to the CPU Or highest slot if you look

which slot game pays out the most use in your PCWorkstation 2) Find a method for using PCIe Slots Underneath your GPU! 3

which slot does fabric softener go in Comments mount it on the one closest to the cpu There is an actual difference aside from location, though largely minor imperceptible depending on GPU  slot, pcie x1 slot, pcie x4 slot, pcie x8 slot, which pcie slot to use, does it matter which